abstract：:All phytopathogenic fungi have two catalase-peroxidase paralogues located either intracellularly (KatG1) or extracellularly (KatG2). Here, for the first time a secreted bifunctional, homodimeric catalase-peroxidase (KatG2 from the rice blast fungus Magnaporthe grisea) has been produced heterologously with almost 100% ...

journal_title：Biochimie

authors： Zámocký M,Droghetti E,Bellei M,Gasselhuber B,Pabst M,Furtmüller PG,Battistuzzi G,Smulevich G,Obinger C

更新日期：2012-03-01 00:00:00

abstract：:Nitroxyl (HNO) is a molecule of significant interest due to its unique pharmacological properties, particularly within the cardiovascular system. A large portion of HNO biological effects can be attributed to its reactivity with protein thiols, where it can generate disulfide bonds. Evidence from studies in erythrocyt...

journal_title：Biochimie

authors： Salie MJ,Oram DS,Kuipers DP,Scripture JP,Chenge J,MacDonald GJ,Louters LL

更新日期：2012-03-01 00:00:00

abstract：:Ceramide synthases (CerSs) are key enzymes in the biosynthesis of ceramides and display a group of at least six different isoenzymes (CerS1-6). Ceramides itself are bioactive molecules. Ceramides with different N-acyl side chains (C(14:0)-Cer - C(26:0)-Cer) possess distinct roles in cell signaling. Therefore, the sele...

journal_title：Biochimie

authors： Schiffmann S,Hartmann D,Fuchs S,Birod K,Ferreiròs N,Schreiber Y,Zivkovic A,Geisslinger G,Grösch S,Stark H

更新日期：2012-02-01 00:00:00

abstract：:Nicotinate phosphoribosyltransferase (NaPRT, EC 2.4.2.11) catalyzes the conversion of nicotinate (Na) to nicotinate mononucleotide, the first reaction of the Preiss-Handler pathway for the biosynthesis of NAD(+). Even though NaPRT activity has been described to be responsible for the ability of Na to increase NAD(+) l...

journal_title：Biochimie

authors： Galassi L,Di Stefano M,Brunetti L,Orsomando G,Amici A,Ruggieri S,Magni G

更新日期：2012-02-01 00:00:00

abstract：:The human liver fatty acid binding protein (hFABP1) participates in cellular long-chain fatty acid trafficking and regulation of lipid metabolism and changes in hFABP1 are associated with an increased risk for type 2 diabetes, cardiovascular disease (CVD), and metabolic syndromes. Gene regulation of hFABP1 is not full...

journal_title：Biochimie

authors： Wu YL,Peng XE,Wang D,Chen WN,Lin X

更新日期：2012-02-01 00:00:00

abstract：:Embryonic stem cells (ESCs) are capable of unlimited self-renewal and differentiation into multiple cell types. Recent large-scale analyses have identified various cell surface molecules on ESCs. Some of them are considered to be beneficial markers for characterization of cellular phenotypes and/or play an essential r...

journal_title：Biochimie

authors： Iwagawa T,Ohuchi SP,Watanabe S,Nakamura Y

更新日期：2012-01-01 00:00:00

abstract：:The hypothesis is proposed that pre-biotic bacterial cell(s) and the first cells capable of growth/division did not require a cytoplasmic membrane. A gel-like microscopic structure less than a cubic micrometer may have had a dual role as both an ancient pre-cytoplasm and a boundary layer to the higher-entropy external...

journal_title：Biochimie

authors： Trevors JT,Pollack GH

更新日期：2012-01-01 00:00:00

abstract：:Structural and motional features in the denatured state of a protein dictate the early folding events starting from that state and these features vary depending upon the nature of the denaturant used. Here, we have attempted to decipher the early events in the folding of Dynein Light Chain protein (DLC8), starting fro...

journal_title：Biochimie

authors： Chakraborty S,Mohan PM,Hosur RV

更新日期：2012-01-01 00:00:00

abstract：:Epithelial Ca(2+)-regulation, which governs cornified envelope formation in the skin epidermis and hair follicles, closely coincides with the expression of S100A3, filaggrin and trichohyalin, and the post-translational modification of these proteins by Ca(2+)-dependent peptidylarginine deiminases. This review summariz...

journal_title：Biochimie

authors： Kizawa K,Takahara H,Unno M,Heizmann CW

更新日期：2011-12-01 00:00:00

abstract：:Posttranscriptional gene regulation by small RNAs and its crucial impact on development, apoptosis, stem cell self-renewal and differentiation gained tremendous scientific attention since the discovery of RNA interference (RNAi) and microRNAs (miRNAs). However, in the last few years, many more examples for regulatory ...

journal_title：Biochimie

authors： Röther S,Meister G

更新日期：2011-11-01 00:00:00

abstract：:Seryl-tRNA synthetases (SerRS) covalently attach serine to cognate tRNA(Ser). Atypical SerRSs, considerably different from canonical enzymes, have been found in methanogenic archaea. A crystal structure of methanogenic-type SerRS revealed a motif within the active site (serine ordering loop; SOL), which undergoes a no...

journal_title：Biochimie

authors： Dulic M,Pozar J,Bilokapic S,Weygand-Durasevic I,Gruic-Sovulj I

更新日期：2011-10-01 00:00:00

abstract：:Sialic acid, a nine-carbon sugar acid usually is present in the non-reducing terminal position of free oligosaccharides and glycoconjugates. Sialylated conjugates in mammals perform important roles in cellular recognition, signaling, host-pathogen interaction and neuronal development. Metabolism of sialylated conjugat...

journal_title：Biochimie

authors： Wickramasinghe S,Medrano JF

更新日期：2011-10-01 00:00:00

abstract：:(-)-Epigallocatechin-3-gallate (EGCG), a well-known chemopreventive factor, induces cancer cells undergoing apoptosis. Over the last several years, we have shown that the mitochondrial NADP(+)-dependent isocitrate dehydrogenase (IDPm) functions as an antioxidant and anti-apoptotic protein by supplying NADPH to antioxi...

journal_title：Biochimie

authors： Kil IS,Jung KH,Nam WS,Park JW

更新日期：2011-10-01 00:00:00

abstract：:As the medaka is a popular fish model in genetics, developmental biology and toxicology, the development of an efficient transgenic medaka technique is important for a variety of biological experiments. Here we demonstrated that the maize transposon system, Ac/Ds, greatly improved the transgenesis of microinjected DNA...

journal_title：Biochimie

authors： Boon Ng GH,Gong Z

更新日期：2011-10-01 00:00:00

abstract：:Pleurocidin (GWGSFFKKAAHVGKHVGKAALTHYL-NH(2)), found in skin mucous secretions of the winter flounder Pleuronectes americanus, is known to possess a high potency and broad-spectrum antimicrobial peptide without cytotoxicity. In this study, to investigate the impact of pleurocidin on apoptotic progress, we observed mor...

journal_title：Biochimie

authors： Cho J,Lee DG

更新日期：2011-10-01 00:00:00

abstract：:When colonizing the digestive tract of mono-associated rats, Ruminococcus gnavus E1 - a bacterium isolated from human faeces - produced a trypsin-dependent anti-Clostridium perfringens substance collectively named Ruminococcin C (RumC). RumC was isolated from the caecal contents of E1-monocontaminated rats and found t...

journal_title：Biochimie

authors： Crost EH,Ajandouz EH,Villard C,Geraert PA,Puigserver A,Fons M

更新日期：2011-09-01 00:00:00

abstract：:From investigation of 2000 soil isolates, we identified two serine-type amidohydrolases that can hydrolyze d-aminoacyl derivatives from the culture supernatant of Streptomyces species 82F2 and 83D12. The enzymes, redesignated as 82F2-DAP and 83D12-DAP, were purified for homogeneity and characterized. Each enzyme had m...

journal_title：Biochimie

authors： Arima J,Ito H,Hatanaka T,Mori N

更新日期：2011-09-01 00:00:00

abstract：:Streptomyces sp. 139 produces a novel exopolysaccharide (EPS) designated Ebosin which can bind IL-1R specifically and exhibits anti-rheumatic arthritis activity in vivo. With the Ebosin biosynthesis gene cluster (ste) consisting of 27 ORFs identified previously the focus of this study was to characterize the protein e...

journal_title：Biochimie

authors： Bai L,Chang M,Shan J,Jiang R,Zhang Y,Zhang R,Li Y

更新日期：2011-09-01 00:00:00

abstract：:The thrombin-binding DNA aptamer (TBA) 5'-d(GGTTGGTGTGGTTGG)-3' forms a G-quadruplex that is necessary for binding to the coagulation factor thrombin. The stability of the G-quadruplex of TBA when bound to thrombin and potassium ion (K(+)) were investigated for the wild-type oligonucleotide and for mutants in which th...

journal_title：Biochimie

authors： Nagatoishi S,Isono N,Tsumoto K,Sugimoto N

更新日期：2011-08-01 00:00:00

abstract：:The present study has employed a combination of spectroscopic, calorimetric and computational methods to explore the binding of the three side-chained triazatruxene derivative, termed azatrux, to a human telomeric G-quadruplex sequence, under conditions of molecular crowding. The binding of azatrux to the tetramolecul...

journal_title：Biochimie

authors： Petraccone L,Fotticchia I,Cummaro A,Pagano B,Ginnari-Satriani L,Haider S,Randazzo A,Novellino E,Neidle S,Giancola C

更新日期：2011-08-01 00:00:00

abstract：:Interleukin-17 (IL-17) is a pro-inflammatory cytokine produced primarily by a subset of CD4(+)T cells, called Th17 cells, that is involved in host defense, inflammation and autoimmune disorders. The two most structurally related IL-17 family members, IL-17A and IL-17F, form homodimeric (IL-17A/A, IL-17F/F) and heterod...

journal_title：Biochimie

authors： Adachi H,Ishiguro A,Hamada M,Sakota E,Asai K,Nakamura Y

更新日期：2011-07-01 00:00:00

abstract：:Ceramidase plays an important role in regulating the metabolism of sphingolipids, such as ceramide, sphingosine (SPH), and sphingosine-1-phosphate (S1P), by controlling the hydrolysis of ceramide. Here we report the cloning and biochemical characterization of a neutral ceramidase from the red flour beetle Tribolium ca...

journal_title：Biochimie

authors： Zhou Y,Lin XW,Yang Q,Zhang YR,Yuan JQ,Lin XD,Xu R,Cheng J,Mao C,Zhu ZR

更新日期：2011-07-01 00:00:00

abstract：:Pseudouridine [Ψ] is a frequent base modification in the ribosomal RNA [rRNA] and may be involved in the modulation of the conformational flexibility of rRNA helix-loop structures during protein synthesis. Helix 69 of 23S rRNA contains pseudouridines at the positions 1911, 1915 and 1917 which are formed by the helix 6...

journal_title：Biochimie

authors： Kipper K,Sild S,Hetényi C,Remme J,Liiv A

更新日期：2011-05-01 00:00:00

abstract：:Cell-to-cell movement of Poa semilatent virus (genus Hordeivirus) in infected plants is mediated by three viral 'triple gene block' (TGB) proteins. One of those termed TGBp3 is an integral membrane protein essential for intracellular transport of other TGB proteins and viral genomic RNA to plasmodesmata. TGBp3 targeti...

journal_title：Biochimie

authors： Shemyakina EA,Erokhina TN,Gorshkova EN,Schiemann J,Solovyev AG,Morozov SY

更新日期：2011-04-01 00:00:00

abstract：:Leishmania parasites cause two million new cases of leishmaniasis each year with several hundreds of millions of people at risk. Due to the paucity and shortcomings of available drugs, we have undertaken the crystal structure determination of a key enzyme from Leishmania major in hopes of creating a platform for the r...

journal_title：Biochimie

authors： Larson ET,Kim JE,Zucker FH,Kelley A,Mueller N,Napuli AJ,Verlinde CL,Fan E,Buckner FS,Van Voorhis WC,Merritt EA,Hol WG

更新日期：2011-03-01 00:00:00

abstract：:C-type lectins are calcium-dependent sugar binding proteins and are distributed ubiquitously amongst vertebrate organisms. As part of a wider study on Australian snake venom components, we have identified and characterised a C-type lectin from the venom of Oxyuranus scutellatus (Australian coastal taipan) with mannose...

journal_title：Biochimie

authors： Earl ST,Robson J,Trabi M,de Jersey J,Masci PP,Lavin MF

更新日期：2011-03-01 00:00:00

abstract：:The human ribosomal protein SA, known also as a precursor of the cell-surface laminin receptor, LAMR, is a protein of the 40S ribosomal subunit. It is homologous to eubacterial ribosomal protein S2p, but has a eukaryote-specific C-terminal domain (CTD) that is responsible in LAMR for the binding of laminin as well as ...

journal_title：Biochimie

authors： Malygin AA,Babaylova ES,Loktev VB,Karpova GG

更新日期：2011-03-01 00:00:00

abstract：:Tetramolecular G-quadruplexes result from the association of four guanine-rich strands. Modification of the backbone strand or the guanine bases of the oligonucleotide may improve stability or introduce new functionalities. In this regard, the 8 position of a guanosine is particularly suitable for introduction of modi...

journal_title：Biochimie

authors： Tran PL,Virgilio A,Esposito V,Citarella G,Mergny JL,Galeone A

更新日期：2011-03-01 00:00:00

abstract：:Human matrilysin-1 (MMP-7) is one of the most potent elastases besides macrophage elastase in the family of matrix metalloproteinases (MMPs). It has been reported to provide macrophages with the highest elastinolytic capacity and plays key roles in diseases such as emphysema and cancer. Describing the enzymatic turnov...

journal_title：Biochimie

authors： Heinz A,Taddese S,Sippl W,Neubert RH,Schmelzer CE

更新日期：2011-02-01 00:00:00

abstract：:The structural difference of flavonoids strongly affects the binding process with plasma proteins. This work in here mainly concerns about the molecular property-binding affinity relationship of dietary flavonoids for common rat plasma proteins (CRPP). The magnitudes of binding constants between flavonoids and CRPP we...

journal_title：Biochimie

authors： Xiao J,Cao H,Chen T,Yang F,Liu C,Xu X

更新日期：2011-02-01 00:00:00

abstract：:Betaine aldehyde dehydrogenase from the human opportunistic pathogen Pseudomonas aeruginosa (PaBADH) catalyzes the irreversible, NAD(P)(+)-dependent oxidation of betaine aldehyde, producing glycine betaine, an osmoprotectant. PaBADH participates in the catabolism of choline and likely in the defense against the osmoti...

journal_title：Biochimie

authors： Zaldívar-Machorro VJ,López-Ortiz M,Demare P,Regla I,Muñoz-Clares RA

更新日期：2011-02-01 00:00:00

abstract：:Organisms exposed to ionizing radiation (IR) undergo increases in the production of reactive oxygen species (ROS), which are determinant components in the induction of apoptosis. Sensitive to apoptosis gene (SAG) encodes a redox-inducible and apoptosis-protective antioxidant protein. This report demonstrates that the ...

journal_title：Biochimie

authors： Kim SY,Yang ES,Lee YS,Lee J,Park JW

更新日期：2011-02-01 00:00:00

abstract：:The eosinophil cationic protein (ECP) is a human antimicrobial protein involved in the host immune defense that belongs to the pancreatic RNase A family. ECP displays a wide range of antipathogen activities. The protein is highly cationic and its bactericidal activity is dependant on both cationic and hydrophobic surf...

journal_title：Biochimie

authors： Sánchez D,Moussaoui M,Carreras E,Torrent M,Nogués V,Boix E

更新日期：2011-02-01 00:00:00

abstract：:Evidence from clinical, animal and cell culture studies demonstrates that increased autotaxin (ATX) expression is responsible for enhancing tumor progression, cell migration, metastases, angiogenesis and chemo-resistance. These effects depend mainly on the rapid formation of lysophosphatidate (LPA) by ATX. Circulating...

journal_title：Biochimie

authors： Samadi N,Bekele R,Capatos D,Venkatraman G,Sariahmetoglu M,Brindley DN

更新日期：2011-01-01 00:00:00

abstract：:The fatty acid composition of animal products (eggs, milk and meat) is the reflect of both the tissue fatty acid biosynthesis and the fatty acid composition of ingested lipids. This relationship is stronger in monogastrics (pigs, poultry and rabbits) than in ruminants, where dietary fatty acids are hydrogenated in the...

journal_title：Biochimie

authors： Kouba M,Mourot J

更新日期：2011-01-01 00:00:00

abstract：:Canine S100A12 (cS100A12) is a calcium-binding protein of the S100 superfamily of EF-hand proteins, and its expression is restricted to neutrophils and monocytes. Interaction of S100A12 with the receptor for advanced glycation end products (RAGE) has been suggested to play a central role in inflammation. Moreover, S10...

journal_title：Biochimie

authors： Heilmann RM,Suchodolski JS,Steiner JM

更新日期：2010-12-01 00:00:00

abstract：:The open reading frame PA3859 of Pseudomonas aeruginosa encodes an intracellular carboxylesterase belonging to a group of microbial enzymes (EC 3.1.1.1) that catalyze the hydrolysis of aliphatic and aromatic esters with a broad substrate specificity. With few exceptions, for this class of enzymes, belonging to the α/β...

journal_title：Biochimie

authors： Pesaresi A,Lamba D

更新日期：2010-12-01 00:00:00

abstract：:Human Rad51 (HsRad51), a key element of the homologous recombination repair pathway, is related to the resistance of cancer cells to chemo- and radio-therapies. This protein is thus a good target for the development of anti-cancer treatments. We have searched for new inhibitors directed against HsRad51 using the Syste...

journal_title：Biochimie

authors： Martinez SF,Renodon-Cornière A,Nomme J,Eveillard D,Fleury F,Takahashi M,Weigel P

更新日期：2010-12-01 00:00:00

abstract：:The kinetoplastid protozoan Leishmania encodes major surface glycoprotein GP63, a zinc metallo-peptidase (EC.3.4.24.36) expressed both in promastigote and amastigote life stages. In the present study, we explored for the first time the role of C-terminal domain (CTD) in proteinase activity by serial truncation of Leis...

journal_title：Biochimie

authors： Mazumder S,Ganguly A,Ali N

更新日期：2010-12-01 00:00:00

abstract：:The lysosomal aspartic protease cathepsin D (cath-D) is overexpressed and hyper-secreted by epithelial breast cancer cells. This protease is an independent marker of poor prognosis in breast cancer as it is correlated with the incidence of clinical metastasis. In normal cells, cath-D is localized in intracellular vesi...

journal_title：Biochimie

authors： Masson O,Bach AS,Derocq D,Prébois C,Laurent-Matha V,Pattingre S,Liaudet-Coopman E

更新日期：2010-11-01 00:00:00